{"id":164602,"date":"2024-04-26T09:55:25","date_gmt":"2024-04-26T07:55:25","guid":{"rendered":"https:\/\/stratec-med.com\/literatur\/effects-of-oxidation-on-the-power-of-chemically-skinned-rat-soleus-fibres-2\/"},"modified":"2024-04-26T09:55:26","modified_gmt":"2024-04-26T07:55:26","slug":"effects-of-oxidation-on-the-power-of-chemically-skinned-rat-soleus-fibres-2","status":"publish","type":"literatur","link":"https:\/\/stratec-med.com\/en\/literature\/effects-of-oxidation-on-the-power-of-chemically-skinned-rat-soleus-fibres-2\/","title":{"rendered":"Effects of oxidation on the power of chemically skinned rat soleus fibres"},"content":{"rendered":"<p>Oxidation alters calcium sensitivity, and decreases maximum isometric force (Po) and shortening velocity (Vmax) of single muscle fibres. To examine the effect of oxidation on the curvature of the force-velocity relationship, which determines muscle power in addition to Po and Vmax, skinned rat type I fibres were maximally activated at 15\u00b0C in a solution with pCa 4.5 and subjected to isotonic contractions before and after 4-min incubation in 50 mM H(2)O(2) (n=10) or normal relaxing solution (n=3). In five oxidised and four control fibres the rate of force redevelopment (ktr), following a rapid release and re-stretch, was measured. This gives a measure of the sum of the rate constants for cross-bridge attachment (f) and detachment (g(1)): (f+g(1)). H(2)O(2) reduced Po, Vmax and ktr by 19%, 21% and 24% respectively (P<0.001), while the shape of the force-velocity relationship was unchanged. Fitting data to the Huxley cross-bridge model suggested that oxidation decreased both the rate constant for cross-bridge attachment (f), and detachment of negatively strained cross-bridges (g(2)), similar to the effect of reduced activation. This suggests that oxidative modification is a possible cause of the variation in contractile properties between muscle fibres of the same type.\n<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Oxidation alters calcium sensitivity, and decreases maximum isometric force (Po) and shortening velocity (Vmax) of single muscle fibres. To examine<\/p>\n","protected":false},"author":1,"parent":0,"menu_order":0,"template":"","format":"standard","meta":{"_acf_changed":false},"tags":[],"thema":[5870],"produktgruppe":[5827],"literatur_kategorie":[7213],"class_list":["post-164602","literatur","type-literatur","status-publish","format-standard","hentry","thema-basics","produktgruppe-leonardo-en","literatur_kategorie-scientific-publications"],"acf":[],"_links":{"self":[{"href":"https:\/\/stratec-med.com\/en\/wp-json\/wp\/v2\/literatur\/164602","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/stratec-med.com\/en\/wp-json\/wp\/v2\/literatur"}],"about":[{"href":"https:\/\/stratec-med.com\/en\/wp-json\/wp\/v2\/types\/literatur"}],"author":[{"embeddable":true,"href":"https:\/\/stratec-med.com\/en\/wp-json\/wp\/v2\/users\/1"}],"version-history":[{"count":0,"href":"https:\/\/stratec-med.com\/en\/wp-json\/wp\/v2\/literatur\/164602\/revisions"}],"wp:attachment":[{"href":"https:\/\/stratec-med.com\/en\/wp-json\/wp\/v2\/media?parent=164602"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/stratec-med.com\/en\/wp-json\/wp\/v2\/tags?post=164602"},{"taxonomy":"thema","embeddable":true,"href":"https:\/\/stratec-med.com\/en\/wp-json\/wp\/v2\/thema?post=164602"},{"taxonomy":"produktgruppe","embeddable":true,"href":"https:\/\/stratec-med.com\/en\/wp-json\/wp\/v2\/produktgruppe?post=164602"},{"taxonomy":"literatur_kategorie","embeddable":true,"href":"https:\/\/stratec-med.com\/en\/wp-json\/wp\/v2\/literatur_kategorie?post=164602"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}